NEB/NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®/96 reactions/E7645L,NEB,,NEB,New England Biolabs,纽英伦,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：NEB/NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®/96 reactions/E7645L

产品编号：E7645L

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>其他>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： NEB

公司：New England Biolabs

公司分类：

商品介绍

The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities.The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.

Now also available with optional SPRIselect® beadsfor clean-up and size-selection steps.Advantages

Get more of what you need, with the highest library yields
Use to generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg
Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples
Improve yields and quality for target enrichmentapplications
Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats

To learn more about how NEBNext Ultra II addresses lower input amounts and challenging sample types, download our technical note.

For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.

A: Libraries were prepared from Human NA19240 genomic DNA using the input amounts and numbers of PCR cycles shown. Manufacturers’ recommendations were followed, with the exception that size selection was omitted.B: Libraries were prepared from Human NA19240 genomic DNA using the input amounts and library prep kits shown without an amplification step, following manufacturers" recommendations. qPCR was used to quantitate adaptor-ligated molecules, and quantitation values were then normalized to the conversion rate for Ultra II. The Ultra II Kit produces the highest rate of conversion to adaptor-ligated molecules, for a broad range of input amounts.

View additional data on library yield

Libraries were made using 500 pg, 1 ng and 100 ng of the genomic DNAs shown and the Ultra II DNA Library Prep Kit and sequenced on an Illumina MiSeq^®. Reads were mapped using Bowtie 2.2.4 and GC coverage information was calculated using Picard"s CollectGCBiasMetrics (v1.117). Expected normalized coverage of 1.0 is indicated by the horizontal grey line, the number of 100 bp regions at each GC% is indicated by the vertical grey bars, and the colored lines represent the normalized coverage for each library.

View additional data on library quality

Libraries were generated from 100 ng of Human NA19240 genomic DNA using the library prep kits shown, following manufacturers" recommendations, and with no amplification step. Library yields were determined by qPCR using the NEBNext Library Quant Kit for Illumina. The NEBNext Ultra II Kit produces the highest yields.

NEBNext Ultra II libraries provide the highest quality sequence data in PCR-free workflows

LIBRARY KIT	TOTAL READS	% DUPLICATION	% MAPPED	% CHIMERAS
Ultra II	3,685,029	0.02	97.00	1.10
Kapa Hyper	3,679,136	0.02	97.00	2.00
TruSeq PCR-Free	3,681,643	0.05	96.65	1.38

PCR-free libraries were generated from 100 ng of Human NA19240 genomic DNA using library prep kits shown, following manufacturers’ recommendations, and with no amplification step. Libraries were sequenced on the Illumina NextSeq 500. Reads were mapped to the GRCh37 reference using Bowtie 2.2.4.This data illustrates that NEBNext Ultra II DNA Library Prep Kit enables high quality sequence data in PCR-free workflows, even with low input amounts.% Mapped:The percentage of reads mapped to human GRCh37 reference.% Duplication:The percentage of mapped sequence that is marked as duplicate.% Chimeras:The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes. NEBNext Ultra II enables construction of high quality libraries from FFPE DNA samples

FFPE DNA	DNA INPUT (ng)	LIBRARY YIELDS IN ng	% MAPPED	% MAPPED IN PAIRS	% DUPLICATION	% CHIMERAS
Kidney Tumor	17	132	91.5	96.1	0.48	3.0
Lung Tumor	20	232	90.1	94.9	0.42	4.1
Liver Normal	20	691	92.6	94.7	0.33	8.6
Breast Tumor	30	514	91.9	95.1	0.37	4.5

Libraries were prepared from 17–30 ng of human DNA extracted from the FFPE tissue samples listed, amplified using 10 cycles of PCR and sequenced on the Illumina MiSeq. Reads were mapped to the GRCh37 reference genome using Bowtie 2.2.4.% Mapped:The percentage of reads mapped to Human GRCh37 reference.% Mapped in Pairs:The percentage of reads whose mate pair was also aligned to the reference.% Duplication:The percentage of mapped sequence that is marked as duplicate.% Chimeras:The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes.This data illustrates that the NEBNext Ultra II DNA Library Prep Kit for Illumina enables high quality sequence data, even with low input amounts of FFPE DNA. View additional data from FFPE DNA samples

For Nimblegen^® enrichment, libraries were prepared with 100 ng of Human HG02922 genomic DNA, using NEBNext Ultra II with the NEBNext Adaptor and index primers, or the Kapa Library Preparation Kit Illumina Platforms with the SeqCap^® Adapter Kit (Roche). Target enrichment was performed with NimbleGen SeqCap Human Exome v3.For SureSelect^XT enrichment, libraries were prepared with 200 ng of Human HG02922 genomic DNA, using NEBNext Ultra II or Kapa Hyper with the NEBNext Adaptor and index primers, or the SureSelect^XT Reagent Kit, Illumina (ILM) platforms with Herculase^® II Fusion DNA Polymerase. Target enrichment was performed with SureSelect^® Human All Exon V6.Manufacturers’ recommendations were followed, including use of the recommended number of PCR cycles for pre- and post-capture. NEBNext Ultra II libraries resulted in the highest post-capture yields for both enrichment workflows.

View additional data on target enrichment

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

This product is related to the following categories:: DNA Library Prep for Illumina

品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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