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主要产品：NEB、内切酶、基因表达、RNAi DNA修饰酶、各种载体、 Marker 、引物、测序 等等
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℡ 4000-520-616
NEB/NEB® PCR Cloning Kit/20 reactions/E1202S
产品编号：E1202S
市  场 价：¥0.00
场      地：美国(厂家直采)
产品分类： 分子类>核酸酶类>其他>
联系QQ：1570468124
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品      牌： NEB
公      司：New England Biolabs
公司分类：
NEB/NEB® PCR Cloning Kit/20 reactions/E1202S
商品介绍

This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. It allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are performed with proofreading DNA polymerases, such as Q5® which produce blunt ends; or nonproofreading DNA polymerases, such as Taq or Taq mixes (OneTaq®, LongAmp® Taq) which produce single base overhangs. This is possible due to “invisible” end polishing components in the master mix that are active during the ligation step only if needed. The kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5´-phosphate groups. To learn more about how the kit works, please view our video.

The ultimate inflexibility:clone with any amplicon made with anyDNA polymerase, with or without 5´ phosphates, purified or not!
PCR cloning withlow/no background
A 500 bp PCR product incubated with the linearized vector in a 3:1 ratioaccording to recommended protocol. 2 μl of reaction was transformed intoprovided NEB 10-beta Competent E. coli and 1/20th of the outgrowth wasplated. Left plate serves as the control, with vector backbone only, right platecontains PCR insert.
Cloning Kit Protocol OverviewProtocol
pMiniT 2.0 Vector Map
Map shown above displays the construct formed if no insert is present. Unique restriction sites are shownin black. Additional restriction sites that can be used for subcloning are shown in red. Expanded box belowshows location of sequencing primers, restriction sites for subcloning, and placement of insertion sitewithin the toxic minigene.
This product is related to the following categories:
DNA Assembly, Cloning and Mutagenesis Kits Products
品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、 产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持 业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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