NEB/Tth Argonaute (TtAgo)/50 pmol/M0665S,NEB,,NEB,New England Biolabs,纽英伦,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：NEB/Tth Argonaute (TtAgo)/50 pmol/M0665S

产品编号：M0665S

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>其他>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： NEB

公司：New England Biolabs

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商品介绍

TtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5′-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg²⁺ (or Mn²⁺) (See product usage note #1)metal ions for the reaction to occur and cleaves a complementary substrate between the bases corresponding to positions 10 and 11 of the DNA guide.

TtAgo is active from 65–85°C (See product usage note #2) and exhibits high activity on single-stranded DNA, moderate to high activity on double-stranded DNA, and mild activity on single-stranded RNA substrates. TtAgo does not introduce double-strand breaks in double-stranded DNA substrates when provided with a single guide, and instead only introduces a nick in the strand complementary to the guide. Activity is not observed on double-stranded RNA substrates. The corresponding 5ʹ and 3ʹ ends of cleavage products are compatible with downstream ligation.⁽¹⁾ It has been demonstrated that in some cases, the addition of thermostable single-stranded DNA binding protein (ET SSB, NEB #M2401) and/or a thermostable helicase can aid in the cleavage of some dsDNA substrates.In the absence of a guide, the apo-form of TtAgo can degrade dsDNA through a process known as “chopping,” which is thought to be the mechanism by which TtAgo naturally generates guides from invading or foreign DNA.⁽²⁾To prevent this non-specific activity, guide should be kept in an approximately 5X molar excess over TtAgo. Additionally, a pre-loading step can be performed in which guide is incubated with TtAgo at ~75°C for 10-30 mins to form the nucleoprotein complex prior to substrate addition. Please see the Protocols, Manuals & Usage section for more details.TtAgo is recommended for in vitro applications.For larger volume or higher concentration requirements, customized and bulk packaging is available by purchasing through the Customized Solutions department at NEB. Please contact custom@neb.com for further information.

Product Source

Thermus thermophilus argonaute (TtAgo) is purified from an E. coli strain that carries a cloned gene from the Gram-negative, thermophilic bacterium Thermus thermophilus which is expressed as a recombinant N-terminal 6X His-tagged fusion.

This product is related to the following categories:: Argonautes,; Programmable Nucleases

品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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