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New England Biolabs
主要产品:NEB、内切酶、基因表达、RNAi DNA修饰酶、各种载体、 Marker 、引物、测序 等等
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NEB/SwaI/R0604S/10,000 units
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各类上皮细胞培养_实验方法_
zhby-w
2021-07-24
昨日,NatureMethods上发表的一篇论文指出:CRISPR-Cas9基因编辑技术导致数百种意料之外的脱靶突变,该论文在业界引发了不小的轰动,也使该技术遭到了质疑。众所周知,CRISPR-Cas9基因编辑技术凭借其简易、高效和多样化的特点,目前被越来越多的人认为是癌症的“控制中心”,可以用来修复导致失明的基因突变、治疗生物的遗传疾病、甚至通过编辑人类胚胎基因来找出导致不孕和流产的原因。蛋白质数据库中编号为5AXW的金黄色葡萄球菌的Cas9蛋白晶状结构然而,昨日发表的论文联合作者、哥伦比亚大学医学中心的StephenTsang博士说,“我们一致认为,CRISPR引起的偏靶突变这一发现具有非常严重的潜在危害,其中包括单核苷酸突变和基因组非编码区域的突变。”在这项研究中,研究人员对课题组此前获得的进行过CRISPR基因编辑的小鼠进行了全基因组测序,并进行了健康程度比对。他们发现:CRISPR基因编辑技术确实成功修复了小鼠体内导致失明的基因,但通过全基因组测序后发现小鼠体内有超过1500个单核苷酸发生突变,并且有100个以上的位点发生发生了大片段的缺失和插入。同时,Tsang研究团队的报告指出:上述这些突变无一通过计算机设置的算法预测到。NatureMethod论文中的部分关键实验结果统计StephenTsang博士进一步说道,“研究人员一般并不会使用全基因组测序手段去检测脱靶效应导致的基因突变位点,并且可能忽视了潜在的重要突变位点,因为即便是单碱基突变也有可能造成较大的影响。我们希望,这一研究结果能够鼓励其他人使用全基因组测序作为确定CRISPR技术所有脱靶效应的方法,从而达到更安全、更精准的基因编辑”。当谈及对于CRISPR技术用于疾病治疗的前景,该研究的共同通讯作者VinitMahajan博士说:“虽然我们依然对CRISPR持乐观态度。但作为医生,我们知道,每一种新疗法都有一些潜在的副作用,我们需要知道这些副作用是什么。”参考资料:https://www.sciencealert.com/turns-out-crispr-gene-editing-can-cause-hundreds-of-unexpected-mutationshttps://eurekalert.org/pub_releases/2017-05/cumc-cge052617.phphttps://phys.org/news/2017-05-crispr-gene-hundreds-unintended-mutations.htmlhttps://phys.org/news/2017-04-accurate-dna-method.html
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指亚基间亚基间吧?简单办1品loading buffer加DTT,另加DTT且煮SDS-PAGE,能看点东西.没差异,二硫键亚基内.加DTT跑量比加,二硫键亚基间.
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NEB公司成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,是生产生命科学试剂的领导者。目前,NEB为基因组研究提供最齐全的重组酶和天然酶,并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程,NEB公司作为先驱公司之一,为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备,有一座现代化的发酵中心及设备齐全的实验室,这些实验室主要用于产品生产、
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