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℡ 4000-520-616
℡ 4000-520-616
NEB/NEBNext® Ultra™ DNA Library Prep Kit for Illumina®/96 reactions/E7370L
产品编号:E7370L
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>其他>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
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品      牌: NEB
公      司:New England Biolabs
公司分类:
NEB/NEBNext® Ultra™ DNA Library Prep Kit for Illumina®/96 reactions/E7370L
商品介绍

The NEBNext® Ultra™ DNA Library Prep Kit for Illumina® contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 5 ng – 1 µg input DNA, in a streamlined workflow. Please note that adaptors and primers are not included in the kit and are available separately.Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a library on the Illumina sequencing platform.

Ultra DNA Library Preparation Workflow for IlluminaUltra DNA Library Preparation Workflow for Illumina
NEBNext Ultra™ DNA provides high library yields even with lowinputs and difficult samplesNEBNext Ultra™ DNA provides high library yields even with low inputs and difficult samples
All libraries were prepared with 5 ng of input DNA and run on an Agilent Bioanalyzer®.A. E. coli genomic DNA libraries. 1: Ladder; 2: Library prepared using NEBNext DNALibrary Prep Master Mix Set (E6040); 3: Library prepared using NEBNext Ultra DNALibrary Prep Kit (E7370). With 5 ng inputs, library yields are significantly higher usingthe NEBNext Ultra™ DNA Kit.B. FFPE sample. Human lung tumor genomic DNA from a 66-year old male, extracted froma 10-year old FFPE block (Biochain, Inc.). 1: Library prepared using NEBNext Ultra DNALibrary Prep Kit (E7370). 2: Input DNA (highly degraded FFPE genomic DNA).
Read depth correlation shows consistent high coverage for 5 ng - 1 µg input amountsRead depth correlation shows consistent high coverage for 5 ng - 1 µg input amounts
Libraries were prepared with various amounts of human IMR90 gDNA (5, 50, and 1000 ng) and PCR amplified. A PCR-free library was also prepared with 1000 ng human IMR90 gDNA. Sequence coverage of 10 kb windows of the human genome was analyzed on Galaxy, and Pearson correlation coefficient (R2 values) were calculated using Microsoft® Excel®. All libraries are highly correlated with each other, independent of DNA input as well as PCR amplification. High coverage was achieved for all DNA input amounts.
NEBNext Ultra DNA Kits display strong performance with input amounts as low as 5 ngNEBNext Ultra DNA Kits display strong performance with input amounts as low as 5 ng
Input DNA: IMR90 genomic DNA (human fibroblast).PF Mismatch Rate: The rate of bases mismatching the reference for all bases aligned to the reference sequence.Percent Duplication: The percentage of mapped sequence that is marked as duplicate.Percent Chimeras: The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes.This data illustrates the strong performance of the NEBNext Ultra DNA Library Prep Kit for Illumina, even with 5 ng of human genomic DNA input. Mismatch rate, % duplication and % chimeras are all low, and similar, regardless of input amount.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
This product is related to the following categories:
Next Generation Sequencing Library Preparation
品牌介绍

NEB公司成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,是生产生命科学试剂的领导者。目前,NEB为基因组研究提供最齐全的重组酶和天然酶,并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程,NEB公司作为先驱公司之一,为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备,有一座现代化的发酵中心及设备齐全的实验室,这些实验室主要用于产品生产、质量监控、 产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一,NEB一直专注于内切酶的研究,并保持 业内领先水平。NEB公司一贯坚持以科学为本的原则,公司生产的试剂因其高质量、高性价比享誉世界。NEB(北京)有限公司 New England Biolabs (Beijing) LTD. NEB(北京)有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司,负责处理其在中国内地及香港地区的全部业务,同时承担部分新产品的研发工作。纽英伦生物技术(北京)有限公司成立于2001年11月,为中国生命科学研究者提供一流的产品和专业的技术服务,促进国内外生命科学学术交流,与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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