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主要产品：NEB、内切酶、基因表达、RNAi DNA修饰酶、各种载体、 Marker 、引物、测序 等等
℡ 4000-520-616
℡ 4000-520-616
NEB/Monarch® HMW DNA Extraction Kit for Cells & Blood/50 preps/T3050L
产品编号：T3050L
市  场 价：¥0.00
场      地：美国(厂家直采)
产品分类： 分子类>核酸酶类>其他>
联系QQ：1570468124
电话号码：4000-520-616
邮      箱： info@ebiomall.com
美  元  价：待定
品      牌： NEB
公      司：New England Biolabs
公司分类：
NEB/Monarch® HMW DNA Extraction Kit for Cells & Blood/50 preps/T3050L
商品介绍

The MonarchHMW DNA Extraction Kit for Cells & Blood provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from cultured cells and whole blood. Utilizing an optimized process that combines gentle cell lysis with a tunable fragment length generation followed by precipitation of the extracted DNA onto the surface of large glass beads, the prep proceeds rapidly and utilizes standard laboratory equipment. DNA size ranges from 50-250 kb for the standard protocol and into the Mb range when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For cells, the process time is only 30 min, while blood samples require erythrocyte lysis and are processed in 60 min. Purity ratios of 1.80-1.90 and 2.2-2.5 (A260/A280 and A260/A230, respectively), are easily and reproducibly achievable, and purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing (Oxford Nanopore Technologies® and Pacific Biosciences®), optical mapping (Bionano Genomics®), and linked-read genome assembly.

Validated Sample Types

Cells

  • K293
  • HeLa
  • NIH3T3
  • Jurkat
  • K562 (suspension cells)
  • HCT116
  • A549
  • U5Os
  • HepG2
  • NCI-460
  • SK-N-SH
  • Aa23

Blood

  • Human
  • Mouse
  • Rat (fresh only)
  • Rabbit

  • Pig
  • Horse
  • Cow
  • Rhesus money
  • Goat (fresh only)
  • Sheep (fresh only)
  • Chicken
  • Turkey

Visit ourinput guidelines or more information

Figure 1: Workflow for isolation of HMW DNA from cells using the Monarch HMW DNA Extraction Kit
Figure 2: Workflow for isolation of HMW DNA from blood using the Monarch HMW DNA Extraction Kit
Figure 3: Reproducible Extraction of HMW DNA from Cells and Blood with the Monarch HMW DNA Extraction Kit
DNA extracted with Monarch HMW DNA Extraction Kit for Cells & Blood. 1 x 106 fresh HEK293 cells and 500 µl fresh human blood were used as inputs and for preps performed according to the kit instructions using the agitation speed indicated above the gel lanes. 500 ng of DNA from the replicates was resolved by PFGE (1% agarose gel, 6 V/cm, 13°C for 20 hours, switch times ramped from 0.5–94 seconds on a BioRad® CHEF-DR® III System). Yield and purity ratios of the individual preps are shown in the accompanying tables. Lambda PFG Ladder (NEB #N0341) was used as molecular weight standard.
Figure 4: Use of varying agitation speeds during lysis produces tunable fragment length of extracted HMW genomic DNA from cells and blood
Preps were performed on duplicate aliquots of 1 x 106 HEK 293 cells and 500 ìl fresh human blood. Samples were agitated at the indicated speed during the lysis step to control the fragmentation of the DNA. Equal amounts of DNA from the replicates (cells: 500 ng; blood: 650 ng) were resolved by PFGE (1% agarose gel, 6 V/cm, 13°C for 20 hours, switch times ramped from 0.5–94 seconds on a BioRad CHEF-DR III System). Yield and purity ratios of the individual preps are shown in the accompanying tables. Lambda PFG Ladder and Lambda DNA-Hind III Digest (NEB #N0341 and #N3012) were used as molecular weight standards. Yield, purity ratios and DINs of the individual preps are shown in the accompanying tables.
Figure 5: Linear correlation between DNA yield and input for cell and blood samples
Summarized yield data for HMW DNA preps are shown carried out at 2,000 rpm during lysis, using HEK293 cultured cells and fresh human blood samples from different donors as input material in the corresponding protocols. The starting materials were diluted to 5 different concentrations to cover the entire recommended input range. Cell samples ≤ 5x105 cells and blood samples <500 µl were purified using the recommended volumes for low input samples. Obtained yields show a high degree of linearity over the displayed input range.
This product is related to the following categories:
Genomic DNA Extraction & Purification,
Nucleic Acid Purification Products
品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、 产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持 业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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