NEB/Monarch® RNA Cleanup Kit (10 µg)/100 preps/T2030L,NEB,,NEB,New England Biolabs,纽英伦,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：NEB/Monarch® RNA Cleanup Kit (10 µg)/100 preps/T2030L

产品编号：T2030L

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>其他>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： NEB

公司：New England Biolabs

公司分类：

商品介绍

The Monarch RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

Monarch RNA Cleanup kits are also available for 50 µg (NEB #T2040) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.

Specifications andApplications:

SPECIFICATIONS
RNA Sample Type	Cleanup and concentration of RNA from enzymatic reactions (labeling, capping, in vitrotranscription reactions, DNase I treatment)
Binding Capacity	10 μg
RNA Size Range	≥ 25 nt ( ≥ 15 nt with modified protocol)
Typical Recovery	70–100%
Elution Volume	6–20 µl
Purity	A_260/280 > 1.8 and A_260/230 > 1.8
Protocol Time	5 minutes of spin and incubation time
Compatible Downstream Applications	RT-PCR, Small RNAlibrary prep for NGS, RNA Library Prep for NGS

APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase)	RNA purified by other methods can be further purified
Enzymatic Reaction Cleanup	Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
In vitro Transcription Cleanup	Enzymes and excess NTPs are removed to yield highly pure synthesized RNA
RNA Gel Extraction	Purification of RNA from agarose gels
RNA Fractionation	Fractionation of RNA into small and large RNA pools

rRNA (10 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #2030) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A₂₆₀ as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in as little as 6 µl.

A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A₂₆₀, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.

The Monarch RNA Cleanup Columns (10 µg, NEB #T2037) ensure zero buffer retention and no carryover of contaminants, enabling sample elution in volumes as low as 6 µl. Moreover, ~90% (~5.4 µl) of the minimal elution volume is recovered from the Monarch RNA Cleanup Columns, compared to other competitor kits.

This product is related to the following categories:: RNA Cleanup Products,; RNA Reagents Products,; RNA Extraction & Purification Products,

This product can be used in the following applications:: RNA Purification and Isolation,; RNA Analysis

品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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