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主要产品：NEB、内切酶、基因表达、RNAi DNA修饰酶、各种载体、 Marker 、引物、测序 等等
℡ 4000-520-616
℡ 4000-520-616
NEB/HiScribe® T7 mRNA Kit with CleanCap® Reagent AG/20 reactions/E2080S
产品编号：E2080S
市  场 价：¥0.00
场      地：美国(厂家直采)
产品分类： 分子类>核酸酶类>其他>
联系QQ：1570468124
电话号码：4000-520-616
邮      箱： info@ebiomall.com
美  元  价：待定
品      牌： NEB
公      司：New England Biolabs
公司分类：
NEB/HiScribe® T7 mRNA Kit with CleanCap® Reagent AG/20 reactions/E2080S
商品介绍

Most eukaryotic mRNAs require a 7-methyl guanosine (m7G) cap structure at the 5´-end and a poly(A) tail at the 3′-end for efficient translation. The HiScribe T7 mRNA Kit with CleanCap Reagent AG utilizes an optimized RNA synthesis formulation and trinucleotide cap analog technology for co-transcriptionally capping mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield. By using a DNA template with a T7 promoter sequence followed by an AG initiation sequence and an encoded poly(A) tail, mRNAs can be transcribed with a 5´-m7G Cap-1 structure that is polyadenylated, translationally competent and able to evade the cellular innate immune response.

The HiScribe T7 mRNA Kit with CleanCap Reagent AG is formatted with individual vials of NTPs and CleanCap Reagent AG to allow for partial or complete substitution of modified NTPs, with a total kit yield of 1.8 mg of mRNA. Cap-1 mRNA synthesized from this kit is suitable for many applications, including transfections, microinjections, in vitro translation, preclinical mRNA therapeutic mRNA studies as well as RNA structure and function analysis.

This kit contains sufficient reagents for 20 reactions (20 µl each). Each standard reaction yields ≥ 90 µg of RNA from 1 µg CLuc AG Control Template DNA. Each kit can yield ≥ 1.8 mg RNA.

Figure: 1: CleanCap Reagent AG
FIgure 2: Schematic of CleanCap Reagent AG Promoter and Initiation Sequence
Figure 3: CleanCap Reagent AG results in higher mRNA synthesis yield than the ARCA analog
All reactions were performed with 5 mM CTP, 5 mM UTP and 6 mM ATP. Standard IVT reactions included 5 mM GTP and no cap analog. ARCA reactions contained a 4:1 ratio of ARCA:GTP (4mM:1mM). IVT with CleanCap Reagent AG contained 5 mM GTP and 4 mM CleanCap Reagent AG and was performed according to recommended protocol (Standard mRNA Synthesis, HiScribe T7 mRNA Kit with CleanCap Reagent AG). Reactions were incubated for 2 hours at 37°C, purified and quantified by NanoDrop®.
This product is related to the following categories:
RNA Synthesis Products
This product can be used in the following applications:
In vitro Transcription for RNA Synthesis
品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、 产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持 业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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氧核糖核酸5ˊ寡核苷酸水解酶厂家 仪器交易网 优势销售 TRIDONIC.ATCO,ZRM 20ES/B 400,模块 聚合物微流控芯片的注射成型 儿童系统性红斑狼疮的第一线治疗药物是 A.环磷酰胺B.羟... 请教:关于Alzhermer病的动物模型 实验室建设与采购讨论版... 研究人员找到和致命前列腺癌相关的基因突变 家医在线健康资讯 SP6 RNA Polymerase | NEB Titration of retrovirus 重磅级研究成果解读脊髓损伤修复领域新进展!资讯... 分子生物学实验室常用仪器使用方法与保养 聚焦2020两会仪器代表发出这些声音资讯 Cytometry:EdU检测细胞增殖效果最佳
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