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主要产品:NEB、内切酶、基因表达、RNAi DNA修饰酶、各种载体、 Marker 、引物、测序 等等
℡ 4000-520-616
℡ 4000-520-616
NEB/NEBExpress Cell-free Ecoli Protein Synthesis System/10 reactions/E5360S
产品编号:E5360S
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>其他>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: NEB
公      司:New England Biolabs
公司分类:
NEB/NEBExpress Cell-free Ecoli Protein Synthesis System/10 reactions/E5360S
商品介绍
The NEBExpress® Cell-free E. coli Protein Synthesis System is a coupled transcription/translation system designed to synthesize proteins encoded by a DNA template under the control of a T7 RNA Polymerase promoter. The system offers high expression levels, the ability to produce high molecular weight proteins, scalability, and is cost-effective for high throughput expression applications. The speed and robustness of the system facilitates protein synthesis in applications such as protein engineering, mutagenesis studies and enzyme screening. In addition, it can be used to generate proteins for biophysical and structure-function analyses.The NEBExpress® Cell-free E. coli Protein Synthesis System contains all the components required for protein synthesis, except for the target template DNA. It is a combination of a highly active cell extract from a genetically engineered strain, a reaction buffer, and an optimized T7 RNA Polymerase, which together yield robust expression of a wide variety of protein targets ranging from 17 to 230 KDa.Protein synthesis is achieved with a short incubation and synthesized protein is compatible with downstream purification or analysis by SDS-PAGE, Western Blot or direct functional assay. The novel formulation of this system allows samples to be loaded directly onto SDS-PAGE, without the need for acetone or TCA precipitation. Additionally, synthesized protein can be isolated from the reaction mixture using affinity purification techniques, such as immobilized metal affinity chromatography (IMAC), for further structural and/or functional characterization.
Figure 1: Protein synthesis using the NEBExpress®Cell-free E. coli Protein Synthesis System
50 µl reactions containing 250 ng template DNA were incubated at 37°C for 3 hours. 2 µl of each reaction were analyzed by SDS-PAGE using a 10–20% Tris-glycine gel. The red dot indicates the protein of interest. M = Unstained Protein Standard, Broad range (NEB #P7717), “Neg” = negative control, no DNA.
This product is related to the following categories:
NEBExpress® Cell-free E. coli Protein Synthesis System,
Cell-Free Protein Expression,
Protein Expression
This product can be used in the following applications:
NEBExpress® Cell-free E. coli Protein Synthesis System,
High-throughput cloning and automation solutions,
Cell-Free Protein Expression,
Protein Expression
品牌介绍

NEB公司成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,是生产生命科学试剂的领导者。目前,NEB为基因组研究提供最齐全的重组酶和天然酶,并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程,NEB公司作为先驱公司之一,为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备,有一座现代化的发酵中心及设备齐全的实验室,这些实验室主要用于产品生产、质量监控、 产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一,NEB一直专注于内切酶的研究,并保持 业内领先水平。NEB公司一贯坚持以科学为本的原则,公司生产的试剂因其高质量、高性价比享誉世界。NEB(北京)有限公司 New England Biolabs (Beijing) LTD. NEB(北京)有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司,负责处理其在中国内地及香港地区的全部业务,同时承担部分新产品的研发工作。纽英伦生物技术(北京)有限公司成立于2001年11月,为中国生命科学研究者提供一流的产品和专业的技术服务,促进国内外生命科学学术交流,与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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