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主要产品：NEB、内切酶、基因表达、RNAi DNA修饰酶、各种载体、 Marker 、引物、测序 等等
℡ 4000-520-616
℡ 4000-520-616
NEB/PURExpress® Disulfide Bond Enhancer/50 reactions/E6820S
产品编号：E6820S
市  场 价：¥0.00
场      地：美国(厂家直采)
产品分类： 分子类>核酸酶类>其他>
联系QQ：1570468124
电话号码：4000-520-616
邮      箱： info@ebiomall.com
美  元  价：待定
品      牌： NEB
公      司：New England Biolabs
公司分类：
NEB/PURExpress® Disulfide Bond Enhancer/50 reactions/E6820S
商品介绍
The PURExpress® Disulfide Bond Enhancer (PDBE) is a proprietary blend of proteins and buffer components designed to correctly fold target proteins with multiple disulfide bonds produced in PURExpress reactions. Added at the beginning of a reaction, the components promote a proper disulfide bond pattern by assisting with the oxidation of cysteine thiols and correcting mis-oxidized substrates. These enhancements can increase the yield of soluble and functionally active protein.
Figure 1:Figure 1
PURExpress Disulfide Bond Enhancer (PDBE) promotes proper folding of active vtPA, a truncated version of tissue plasminogen activator with 9 disulfide bonds, 8 of which are non-consecutive. (A.) Reactions were set-up according to PURExpress specifications with the vtPA template DNA. After a two-hour incubation at 37°C, 5 µl of each reaction was used in an activity assay and cleavage of the chromogenic substrate was monitored for one hour. (B.) 2.5 µl of each reaction was resolved by SDS-PAGE and the gel stained with Coomassie Blue. The vtPA target protein is marked by a red arrow. The addition of PDBE generates active protein. The difference in observed activity is due to disulfide bond folding, as measured by functional activity of the target, and is not due to differences in the amount of protein produced by PURExpress.
Figure 2:Figure 2
PURExpress Disulfide Bond Enhancer is optimized for use in PURExpress but also shows utility in S30 lysate-based IVTT kits from other suppliers. Reactions were set-up according to manufacturer"s specifications with equivalent amounts of template DNA encoding a truncated version of tissue plasminogen activator (9 disulfide bonds, 8 non-consecutive). After a two-hour incubation, 5 µl of each reaction was used in an activity assay and cleavage of the chromogenic substrate was monitored for one hour.
Figure 3:Figure 3
PURExpress Disulfide Bond Enhancer promotes proper folding of active Gaussia Luciferase (GLuc) which contains 5 probable disulfide bonds. Reactions were set-up according to PURExpress specifications with equivalent amounts of template DNA encoding GLuc. After a two-hour incubation at 37°C, triplicate aliquots (2.5 µl) of each reaction were incubated with 50 µl of the GLuc substrate solution and the relative luminescence recorded after a 4 second counting window.
This product is related to the following categories:
NEBExpress® Cell-free E. coli Protein Synthesis System,
PURExpress,
Cell-Free Protein Expression,
Protein Expression
This product can be used in the following applications:
NEBExpress® Cell-free E. coli Protein Synthesis System,
PURExpress,
Cell-Free Protein Expression,
Disulfide-bonded Protein Expression,
Expression of Difficult Proteins,
Protein Expression
品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、 产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持 业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

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