p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases. The PNPP phosphatase activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to
p-nitrophenol, a chromogenic product with absorbance at 405 nm (1). The assay can be used for the quick analysis of the protein phosphatase activity under any non-standard conditions.The advantage of the PNPP phosphatase activity assay is that unlike radioactive assays the substrate concentration can be much higher than the K
m. The initial velocity can be recorded in the continuous assay, but the assay volume is larger than in a radioactive assay (about 1 ml to fill a 1 ml spetrophotometer cuvette) (1,2). The reaction volume in a single-point assay can be very small because the reaction is stopped with the amount of NaOH enough to fill the cuvette (1,3,4).
- This product is related to the following categories:
- Protein Phosphatases Products
- This product can be used in the following applications:
- Protein Phosphatases and Kinases
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