请使用支持JavaScript的浏览器!
公司首页
公司介绍
产品中心
新闻动态
资料下载
在线留言
联系方式
在线下单
欢迎来到蚂蚁淘！（生命科学科研用品平行进口B2B商城） 请登录 免费注册 询价篮
我的蚂蚁淘 产品分类 联系我们
主要产品：NEB、内切酶、基因表达、RNAi DNA修饰酶、各种载体、 Marker 、引物、测序 等等
℡ 4000-520-616
℡ 4000-520-616
NEB//E7000S/24 reactions
产品编号：E7000S-24reactions
市  场 价：¥82000.00
场      地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮      箱： info@ebiomall.com
美  元  价：$4100.00
品      牌： NEB
公      司：New England Biolabs
公司分类：
NEB//E7000S/24 reactions
商品介绍

Description:







Targetenrichment,coupledwithnextgenerationsequencing(NGS),enableshighthroughput,deepsequencingofgenomicregionsofinterest.NEBNextDirectisanovel,hybridization-basedcapturemethodofferingsignificantadvantagesovertrADItionalin-solutionhybridizationandmultiplexPCRprotocols.
 
IntheNEBNextDirecttargetenrichmentapproach(Figure1),fragmentedDNAisrapidlyhybridizedtobiotinylatedoligonucleotidebaitsthatdefinethe3´endofeachtargetofinterest.Thebait-targethybridsareboundtostreptavidinbeadsandany3´offtargetsequenceisremovedenzymatically.Thiscombinationofashorthybridizationtimewiththeenzymaticremovalof3´offtargetsequenceenablesgreatersequencingefficiencyrelativetoconventionalhybridization-basedenrichmentmethods.ThetrimmedtargetsarethenconvertedintoIllumina-compatIBLelibrariesthatincludeuniquemolecularidentifiers(UMI)andasamplebarcode.Sequence-readylibrariesaregeneratedwithinoneday.Theprocedureiscompatiblewithmostautomatedliquidhandlinginstruments.

TheNEBNextDirectHotSpotCancerPanelcontainsbaitsthatcapturebothstrandsofDNAacross190commoncancertargetsfrom50genes,encompassingapproximately40kbofsequenceandincludingover18,000COSMICfeatures(Table1).Thepanelisdesignedtogeneratetargetsofroughly150bp,compatiblewithPE75Illuminasequencing.

Advantages
  • Generateahigherpercentageofyoursequencingreadsaligningtoyourtargets
  • Eliminatetheneedtoover-sequence,reducingcostpersample
  • Obtainuniformsequencingofalltargets,regardlessofGCcontent
  • Savetimewitha1-dayworkflowthatcombinesenrichmentwithlibrarypreparation
  • GeneratehighqualitylibrarieswithlimitedinputamountsanddegradedDNAsamples,includingFFPEandctDNA
  • Distinguishmolecularduplicates,reducingfalsepositivevariantsandimprovingsensitivity


Figure1.NEBNextDirectemploysafasthybridization-basedworkflowthatcombinescapturewithlibrarypreparation.

fast hybridization workflow


Table1.Targetsincluderegionsfromthefollowingcancer-relatedgenes:

Targets include regions from the following cancer-related genes


Figure2.TheNEBNextDirectCancerHotSpotPaneldemonstratestheABIlitytoaccuratelydetectarangeofnucleicacidvariants.

Allele Frequency
Thisfigureshowstheexpectedversusobservedvariantallelefrequencies(VAF)acrosstherangeofwell-characterizedvariantspresentinapoolof24HapMapsamplesscreenedagainsttheNEBNextDirectCancerHotSpotPanel.100ngofinputDNAwasused,samplesweresequencedontheIllumina®MiSeq®using2x75bpsequencing,andstandarddataanalysisandvariantcallingalgorithmswereused.Wewereabletosuccessfullydetect100%ofthe168truthvariantspresentacrossarangeof2-100%VAF.ThehighdegreeoflinearityacrossthisbroaddynamicrangedemonstratestheabilityoftheNEBNextDirectCancerHotSpotPaneltoaccuratelypredictvariantallelefrequenciesacrossabroaddynamicrange.


Figure3.TheNEBNextDirectCancerHotSpotPaneldeliversahighpercentageofsequencereadsmappingtotargets,evenwithchallengingsampletypes.

The NEBNext Direct Cancer HotSpot Panel delivers a high percentage of sequence reads mapping to targets, even with challenging sample types.

  • Graphshowsthepercentageofalignedsequencereadsthatmaptothetargets
  • 100ngofDNAwasusedforeachlibrarypreparation
  • ReadsweregeneratedonanIllumina®MiSeq®with2x75bpreads,8bpsampleID,and12bpuniquemoleculeID
  • AlignmentswereperformedwithBWA-MEMandPCRduplicateswerefilteredusingtheuniquemoleculeIDs

Figure4.TheNEBNextDirectCancerHotSpotPaneldisplayshighuniformityofcoverageacrosstargets.

Cancer HotSpot Panel displays high uniformity of coverage across targets.

  • Graphshowsthepercentageoftargetbasessequencedtoatleast50%,33%,and25%ofthemeanreaddepth
  • 100ngofDNAwasusedforeachlibrarypreparation
  • ReadsweregeneratedonanIlluminaMiSeqwith2x75bpreads,8bpsampleID,and12bpuniquemoleculeID
  • AlignmentswereperformedwithBWA-MEMandPCRduplicateswerefilteredusingtheuniquemoleculeIDs


Figure5.TheNEBNextDirectCancerHotSpotPaneloffersminimizedbiasacrosssequencecontent.

minimized bias across sequence content

  • GraphshowsthenormalizeddepthofcoverageoftargetsofvaryingGCcontent
  • 100ngofDNAwasusedforeachlibrarypreparation
  • ReadsweregeneratedonanIlluminaMiSeqwith2x75bpreads,8bpsampleID,and12bpuniquemoleculeID
  • AlignmentswereperformedwithBWA-MEMandPCRduplicateswerefilteredusingtheuniquemoleculeIDs

ILLUMINA®andMISEQ®areregisteredtrademarksofIllumina®,Inc.

LotControl

Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage

ReagentsSupplied

Thefollowingreagentsaresuppliedwiththisproduct:

Storeat(°C)Concentration
NEBNextDirect®FFPEPhosphorylationEnzyme-20
NEBNextDirect®FFPEPhosphorylationBuffer-20
NEBNextDirect®HybridizationAdditive-20
NEBNextDirect®dA-TailingEnzyme-20
NEBNextDirect®3´Adaptor-20
NEBNextDirect®Ligase-20
NEBNextDirect®5´BluntingEnzymeMix-20
NEBNextDirect®5´UMIAdaptor-20
NEBNextDirect®CleavingEnzymeMix-20
NEBNextDirect®Q5MasterMix-20
NEBNextIndexPrimerMixD01-D08(E7020-E7027)-20
NEBNextDirect®BeadWash125
NEBNextDirect®StreptavidinBeads4
NEBNextDirect®HybridizationWash(HW)4
NEBNextDirect®BeadPrepBuffer4
NEBNextDirect®dA-TailingBuffer4
NEBNextDirect®AdaptorLigationBuffer4
NEBNextDirect®CleavingBuffer4
NEBNextDirect®BeadWash24
NEBNextDirect®3´BluntingEnzymeMix-20
NEBNextDirect®5´BluntingBuffer4
NEBNextDirectCancerHotSpotBaits-20
NEBNextSamplePurificationBeads4
NEBNextDirectHybridizationBuffer4
品牌介绍

NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂的领导者。目前，NEB为基因组研究提供最齐全的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。回顾三十余年来的历程，NEB公司作为先驱公司之一，为促进生物科技工业的发展做出了巨大的贡献。NEB美国总部乔 迁新址后拥有最尖端的设备，有一座现代化的发酵中心及设备齐全的实验室，这些实验室主要用于产品生产、质量监控、 产品开发和基础科研之用。作为首批以商业规模生产限制性内切酶的公司之一，NEB一直专注于内切酶的研究，并保持 业内领先水平。NEB公司一贯坚持以科学为本的原则，公司生产的试剂因其高质量、高性价比享誉世界。NEB（北京）有限公司 New England Biolabs (Beijing) LTD. NEB（北京）有限公司New England Biolabs (Beijing) LTD. 为美国New England Biolabs, Inc.在华投资兴建的独资子公司，负责处理其在中国内地及香港地区的全部业务，同时承担部分新产品的研发工作。纽英伦生物技术（北京）有限公司成立于2001年11月，为中国生命科学研究者提供一流的产品和专业的技术服务，促进国内外生命科学学术交流，与中国科研工作者一起将中国推入到二十一世纪生命科学的前沿中去。

公司介绍
产品分类
Protein Expression|Protein Purification|Protein Tools DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat DNA Modifying Enzymes DNA Assembly Cloning and Mutagenesis Kits DNA Modifying Enzymes and Cloning Technologies|NGS Sample Prep & Target Enrichment|PCR Buffers|Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrich Protein Expression|Protein Expression & Purification Technologies|Protein Purification Epigenetics|Restriction Endonucleases DNA Modifying Enzymes and Cloning Technologies|Epigenetics Nucleic Acid Purification|RNA Reagents Epigenetics DNA Modifying Enzymes and Cloning Technologies Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio Buffers|Markers & Ladders|RNA Reagents Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment Nucleic Acid Purification|Protein Expression & Purification Technologies|RNA Reagents Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno Buffers|DNA Modifying Enzymes and Cloning Technologies Buffers|Competent Cells DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno Buffers Buffers|Nucleic Acid Purification Epigenetics|Protein Expression & Purification Technologies|Protein Purification Epigenetics|Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplific DNA Plasmids & Substrates Markers & Ladders|RNA Reagents Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno DNA Assembly, Cloning and Mutagenesis Kits DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot Competent Cells|Protein Expression & Purification Technologies Protein Tools Epigenetics|Next Generation Sequencing Library Preparation|NGS Sample Prep & Target En Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR Glycobiology|Protein Tools RNA Reagents Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR Protein Expression|Protein Expression & Purification Technologies|Protein Purification Gene Expression DNA Modifying Enzymes and Cloning Technologies|RNA Reagents Cellular Analysis|DNA Plasmids & Substrates|Protein Tools Sample Preparation for Next Generation Sequencing Markers & Ladders|Protein Tools PCR, qPCR & Amplification Technologies DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno Restriction Endonucleases Competent Cells Epigenetics|Next Generation Sequencing Library Preparation|NGS Sample Prep & Target En DNA Modifying Enzymes and Cloning Technologies|Nucleic Acid Purification PCR, Polymerases & Amplification Technologies Protein Expression|Protein Expression & Purification Technologies DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot Cellular Analysis Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec Nucleic Acid Purification Epigenetics|NGS Sample Prep & Target Enrichment|PCR, Polymerases & Amplification T DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat Buffers|Glycobiology Nucleic Acid Purification|Protein Tools NGS Sample Prep & Target Enrichment|PCR, Polymerases & Amplification Technologies| Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno Buffers|Competent Cells|Protein Expression|Protein Expression & Purification Technolog Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR DNA Assembly Cloning and Mutagenesis Kits|DNA Modifying Enzymes and Cloning Technologies Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno Buffers|PCR, Polymerases & Amplification Technologies DNA Modifying Enzymes and Cloning Technologies|Genome Editing Buffers|Next Generation Sequencing Library Preparation Next Generation Sequencing Library Preparation Cellular Analysis|Protein Tools PCR, Polymerases & Amplification Technologies|Protein Expression|Protein Expression &a Genome Editing|RNA Reagents Cellular Analysis|Epigenetics|Protein Tools DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno Protein Expression & Purification Technologies|Protein Purification Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio Competent Cells|Protein Expression|Protein Expression & Purification Technologies DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec Buffers|RNA Reagents Markers & Ladders DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec Nucleic Acid Purification|PCR, Polymerases & Amplification Technologies|RNA Reagents DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno Next Generation Sequencing Library Preparation|RNA Reagents PCR, Polymerases & Amplification Technologies|RNA Reagents Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot Protein Expression & Purification Technologies|RNA Reagents Genome Editing Glycobiology Cellular Analysis|Protein Expression & Purification Technologies|Protein Tools Epigenetics|Next Generation Sequencing Library Preparation DNA Plasmids & Substrates|Protein Expression|Protein Expression & Purification Tec Buffers|Markers & Ladders|Protein Tools DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno Markers & Ladders Markers & Ladders|Nucleic Acid Purification|RNA Reagents Buffers|Restriction Endonucleases Protein Expression & Purification Technologies Buffers|Markers & Ladders
销售排行
问答列表
氧核糖核酸5ˊ寡核苷酸水解酶厂家 仪器交易网 优势销售 TRIDONIC.ATCO,ZRM 20ES/B 400,模块 聚合物微流控芯片的注射成型 儿童系统性红斑狼疮的第一线治疗药物是 A.环磷酰胺B.羟... 请教:关于Alzhermer病的动物模型 实验室建设与采购讨论版... 研究人员找到和致命前列腺癌相关的基因突变 家医在线健康资讯 SP6 RNA Polymerase | NEB Titration of retrovirus 重磅级研究成果解读脊髓损伤修复领域新进展!资讯... 分子生物学实验室常用仪器使用方法与保养 聚焦2020两会仪器代表发出这些声音资讯 Cytometry:EdU检测细胞增殖效果最佳
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关，提供发票
及时交付图标
及时交付
限时必达 不达必赔
快速导航：
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏  0512-67156496（苏州）
版权信息：
©  2015- 苏州蚂蚁淘生物科技有限公司 版权所有 中国
网站备案：
友情链接：
免责声明：
本站有部分内容来自互联网，如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权，请来电或致函告之，
本网站将在规定时间内给予删除等相关处理。
扫描二维码关注官方微信
获取最新促销及产品信息