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Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus.

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NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

High efficiency adaptor ligation Minimized adaptor-dimer formation Includes index primers for library multiplexing Increased sample identification specificity
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
Dual Index Workflow

Lot Control The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page Kit Components

The following reagents are supplied with this product:


Materials Required but not Supplied For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualE7600
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] E7600S_v1
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEBs Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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RTCA技术简介:

原理:实时无标记动态细胞分析技术(RTCA,RealTimeCellularAnalysis)是通过特殊工艺,将微电子细胞传感器芯片整合到细胞检测板的底部,用以构建实时、动态、定量跟踪细胞形态和增殖分化等改变的细胞阻抗检测传感系统。当贴壁生长在微电极表面的细胞引起贴壁电极界面阻抗的改变时,这种改变与细胞的实时功能状态改变呈相关性,通过实时动态的电极阻抗检测可以获得细胞生理功能相关的生物信息,包括细胞生长、伸展、形态变化、死亡和贴壁等。


典型的利用RTCA技术获得的抗体依赖细胞介导的细胞杀伤(ADCC)实验检测结果:

 

  

             实时动态检测IGF-1R抗体介导下NK-92细胞                    实时监测不同效靶比NK-92细胞对A549细胞的

                   对DU-145细胞的杀伤作用(ADCC)                                                    细胞毒性作用

 



乳酸脱氢酶释放法简介:

原理:乳酸脱氢酶(LDH)存在于细胞内,正常情况下,不能透过细胞膜。当细胞受到损伤时,LDH可从细胞内释放至培养液中。释放出来的LDH在催化乳酸生成丙酮酸的过程中,使氧化型辅酶I(NAD+)变成还原型辅酶(NADH),后者再通过递氢体-吩嗪二甲酯硫酸盐(PMS)还原碘硝基氯化氮唑蓝(INT)或硝基氯化四氮唑蓝(NBT)形成有色的甲基化合物,在570nm波长处有一个吸收峰,利用读取的光吸收值,可测得杀伤细胞毒活性。



乳酸脱氢酶释放法实验结果:
 

 

Enables incorporation of two barcodes into each library; includes 8 barcodes for one end and 12 for the other, for a total of 96 possible combinations.